Determination of kinetic parameters of fructosyltransferase by simulated anneaning method

Abstract

Simulated Anneaning (SA) was invented by S. Kirkpatrich, C. D. Gellatt, M. P. Vecchi in the year of 1983. This method is based on Metropolis type. SA is an effective tool for solving problems related to search and optimization, especially those with large dimensional search problems. In this article, SA was used to determinate the kinetic parameters of fructosyltransferase. The method really helped reduce the quantity of experiments, time and cost related to study of kinetics of fructosyltransferase (FTS), especially in case of multiple substrates.