Selection of optimum conditions for culture and fermentation of uricase production by Pseudomonas putida CN3

  • Phạm Thanh Hà
  • Trần Đình Mấn
  • Trần Thị Hoa

Abstract

The optimum culture conditions for uricase production by  Psedomonas putida CN3 in Erlenmeyer conical flasks were examined. The results showed that the optimum temperature was at 30ºC and incubation period required for maximumuricase production was 48 hours with aeration level at 250 rpm and at pH 8.0. 0.5 % uricacid proved to be the best induction substrate, 0.5 % glucose and 0.3 % yeast extract were found tobe the best carbon and nitrogen sources. Production of  uricase of  P. putidaCN3 was conducted in 5L fermenter under controlted conditions indicated that the uricolytic activity reached 1.21 U/ml in 30 hours and specific activity was 1.33 U/mg proteins. P. putida CN3 is a protential strain for uricase producing. 

điểm /   đánh giá
Published
2016-01-18
Section
Articles