ISOLATION, PURIFICATION AND CHARACTERIZATION OF PENICILLIN-RESISTING STAPHYLOCOCCUS AUREUS INHIBITING ANTIBIOTIC FROM STREPTOMYCES SP. QN63

  • Dương Minh Lam
  • Đặng Ngọc Quang
  • Nguyễn Thị Hà

Abstract

The strain Streptomyces sp. QN63 which was isolated from mangrove soil showed high antagonistic ability on penicillin-resisting Staphylococcus aureus. The antibiotic from this strain was thermostable. In this study, we used microbiological and biochemical methods for estimating the antibiotic activity range of the crude antibiotics solution. The isolation, purification and structuralization of the antibiotic were taken. The results showed that the crude antibiotics had wild range of bacterial antagonistic ability (on both Gram positive and Gram negative bacteria) but it did not have and inhibiting activity against tested fungi. The antibiotic composes of two fragments (PĐ1 and PĐ2) in which PĐ2 showed higher bacterial antagonistic ability. The cyclic peptide structure of the PĐ2 was clarified by using both magnetic nuclear resonance of H and 13C and massive charge measurements
điểm /   đánh giá
Published
2016-01-18
Section
Articles