STUDY ON ACTIVATION OF IMMOBILIZED YEAST CELLS AFTER CATALYST TIME IN ALCOHOL FERMENTATION

Abstract

This paper is aimed to study on the activation of immobilized yeast cells after catalyst time. After a defined time of alcol fermentation S.cerevisiae, which immobilized on Ca-Alginate decreased its activity.

To recover the quantity of yeast as well as its quality the solutions were as followings:

- Gel washing after 40 days of fermentation.

- Seed cleaning solution was NaHCO3 0,5 %.

- Shaking at 200 r/min at 300C for 24 hours.

- Luorganic nitrogen and phosphorous sources used were urea of 0,05 % and KH2PO4 of 0,05 %, respechvely.

- The alcol concentration obtained after immobilization of yeast can be reache for 10,7 %.