EFFECT OF ACTIVATED CHARCOAL AND VENTILATION CULTURE ON GROWTH AND DEVELOPMENT OF GERBERA JAMESONII IN VITRO AND EX VITRO

Abstract

Gerbera (Gerbera jamesonii) is one of flower of high economic value and popularity cultural in many countries all over the world. Gerbera is very popular used as pot flower, cut flower or as a decorative garden. The requirements for micropropagation of Gerbera are improving the system and culture medium in order to enhance the quality of in vitro flowers and respond seedling provision on a large-scale. In this study, the effect of activated charcoal and ventilation culture on growth and development of Gerbera in vitro and ex vitro stage were investigated. The results showed that shoots grown and developed well when cultured on MS

medium containing 30 g/l sucrose, 9 g/l agar and 1 g/l activated charcoal in ventilation culture.

However, the position of AC medium layer had little effect on the growth and development of Gerbera in vitro. Plantlets cultured on MS medium supplemented with 1.0 g/l AC in ventilation culture transferred to greenhouse gave a very high survival rate of 95 %.