STUDY ON HYDROGEN PRODUCTION CAPABILITY OF BACTERIAL STRAIN Thermoanaerobacterium aciditolerans Trau DAt ISOLATED FROM VIETNAM IN ANAEROBIC CONDITION

Abstract

Hydrogen is paid of attention not only in the world but also in Vietnam because of its great potential as a clean energy. In this paper, bacterial strain Thermoanaerobacterium aciditolerans Trau DAt isolated in Vietnam has the ability to produce hydrogen in anaerobic condition at 55^oC. Study on effects of inoculum ratio, carbon sources, nitrogen sources, ferrous concentration, pH and salt concentration on the growth and hydrogen production of Trau DAt strain indicated that the suitable condition for its growth and hydrogen production including 10 % started culture on medium contens (g/l) glucose - 10, yeast extract - 3, FeSO₄.7H ₂O - 5, pH 6.5. In suitable condition, Trau DAt strain produces 198 ml gas/600 ml of fermentation solution in anaerobic fermentation at flask scale (volume 600 ml). A GC-TCD analysis showed that hydrogen occupied 42.95 % of total gas in anaerobic fermentation. The obtained results indicated the remarkable potentiality of the Trau DAt strain in application to larger fermentation scale for biohydrogen production by native bacteria in Vietnam.