ADENOVIRUS VECTOR TECHNOLOGY AND POTENTIAL APPLICATION IN TRIGGER OF POUTRY'S IMMUNITY

  • Phạm Việt Cường
  • Nguyễn Thị Kim Cúc

Abstract

Recombinant adenoviruses are versatile tools for gene delivery and expression. Adenovirus vectors were tested as vaccine vehicles in some pre-clinical and clinical studies for infectious diseases such as measles, hepatisis B, rabies, anthrax, Ebola, SARS, HIV-1, malaria, tuberculosis and influenza. With development of genetic engineering techniques, first-, second- and third-adenovirus vector generations have been developed, meeting strict demands of an expression vector. There are numerous examples of successful using adenovirus vectors in human and animal gene therapy. Adenovirus vectors can be used in (i) gene therapy in cancer treatment as such delivery of tumour inhibition gene p53 and p56, antisense DNA, rybozymea and single chain antibody, apoptosis gene, herpes simplex virus thymidine kinase and cytosine deaminase; (ii) gene therapy for genetic diseases.  In this case, adenovirus vectors specially constructed for treatment of cystic fibrosis, lung  diseases; (iii) Supplementary therapy; and (iv) other applications as for the production of a number of proteins for more-detailed molecular analysis. Adenovirus vector has been known to induce both innate and adaptive immune responses. The activation of innate immune system was stimulated by virus particles so is independent of virus DNA reproduction. Adenovirus affect immune and non-immune cells including epithelium and endothelial cells, promoting series of cell signals and thus reinforcing host immune system. Vector - based on human Adenovirus type 5 have been investigated for poultry vaccine production. The scientific reports  document protective immunity against avian influenza (AI) virus has been elicited in chickens by single dose in ovoor i.m. vaccination with a replication-competent adenovirus (Ad)-free human Advector vaccine containing specific antigens of AI. 

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Published
2014-12-02
Section
Articles