Research the micropropagation in vitro and detection rotundine of Stephania Rotunda Lour.

Abstract

Binh voi (Vietnamese name), Stephannia RotundaLour is a wild herbal species often found in jungles of Binh Thuan, Ninh Thuan, Lam Dong and  Dong Nai. This is a valuable medicinal herb that can treat some diseases, such as: enteritis, stomachache, especially depression. Recently, because of the exploitation and destruction of jungles, the Binh Voi will be extinct in near future is undeniable. Therefore, using preservation and restoration techniques is necessary and in vitro is a efficient one. Media have 3 - 10  mg/l of 2,4-D formed callus from cuttings of the  Stephania rotundaLour. The medium having higher concentration of minerals had a higher ability of forming callus. Having 1 mg/l BA and 0,2mg/l of NAA in the medium stimulated the forming of shoots from buds on cuttings. The substance alkaloid rotundine, which can cure the depression, appeared on the stem, bulb (natural) and callus (in vitro) samples.  And the highest concentration of alkaloid rotundine is from bulb samples