Expression and purification of epitope flagellar antigen H:1,2 (FljB1) for diagnosistic salmonella kit development.

Abstract

Salmonella typhimurium  is one of the two main  S. enteritica  serovars causing food poisoning for the human. Until recently, the most common cause of food poisoning by Salmonella is due to  S. typhimurium. The disease is characterized by diarrhea, abdominal cramps, vomiting and nausea, and generally lasts up to 7 days. Unfortunately, in immunocompromized people, that is the elderly, young, or people with depressed immune systems, Salmonella infections are not treated with antibiotics. Salmonella typhimurium  is primarily associated with cattle but it has spread to a range of food animals, including pigs, sheep and poultry. Chickens that survive the initial infection are lifelong carriers of the S. typhimurium and have no sign of an illness, which are very difficult to demonstrate by microscopic examination specifically. Currently, no drug or vaccine is available to clear the  S. typhimurium completely or prevent the chickens from the S. typhimurium infection. Due to the widespread occurrence of S. typhimurium, diagnosis and prevention of that disease is important in areas of both farm and factory. We must anticipate that it may become more widespread in the environment and hence throughout the food chain. We must regard to an 'emerging' pathogen and monitor the situation accordingly. Therefore, it is necessary to develop a reliable, sensitive, specific, and inexpensive immunodiagnosis kit to detect both acute and latent infections with the S. typhimurium