Evaluation of molecular variation of mutated peanutlines by SSR markers

  • Đinh Thị Phòng
  • Ngô Thị Lam Giang

Abstract

Eleven SSR markers were used to analyse molecular variation of 24 mutated peanut lines (eight  originated  from  VD2  variety  and  16  from  L9801  variety).  Overall,  8  out  of  11  used markers revealed polymorphism with Polymorphic Information Content (PIC) values from 0.44 (L41) to 0.85 (L32), in which 7 markers showed the high level of polymorphism with PIC values ≥0.5. The number of amplified DNA fragments ranged from 1 to 9 per marker with sizes ranging approximately from 120 bp to 360 bp. The genetic dissimilarity coefficients analysis showed that among 8 mutated peanut lines, VD2-1-1, VD2-1-2and VD2-1-3 lines were identical but 4% different from VD2 variety (1-0.96). It is 27% (1-0.73) between VD2-2-3 and VD2-2-4 lines, and VD2 variety. Compare 16 mutated peanut lines and the parent variety L9801, the genetic dissimilarity coefficient values ranged from 9% to45%, in which L9801-2-2 line had the least different with about 9% (1-91), L9801-3-4 line was  the most difference in genetic about 45% (1-0,55). L981-1-2 and L9801-2-1 lines, L9801-3-1 and L9801-4-1 lines were identical.

điểm /   đánh giá
Published
2014-10-21
Section
Articles