Effects of Plant Growth Regulators and Micronutrients on In vitro Growth and Flowering in Roses
An in vitro flowering system has been considered as a convenient tool not only to study flowering mechanism but also to produce commercial in vitro flowers. This study evaluated effect of plant growth regulators (BA and TDZ), AgNO3 and CoCl2 on growth and in vitro flowering of three rose varieties (HV, HD, HT). The results showed that all tested compounds had different effects on plant growth but only TDZ and AgNO3 were able to induce flowering. On MS medium containing 0.2 mg/l TDZ, only HV variety was induced flowering with the rate of 72.2%, while on medium supplemented 30 μM AgNO3, after 21-25 days, all three varieties HT, HV and HD were induced flowering with the rates of 30%, 40% and 50%, respectively; the flowers lasted as long as 14-16 days.