Selection of Porcine Circovirus Type 2 (PCV2) Isolates for Development of Vaccine against Postweaning Multisystemic Wasting Syndrome

  • Huỳnh Thị Mỹ Lệ
  • Lê Văn Phan
  • Nguyễn Văn Giáp
  • Trịnh Đình Thâu

Abstract

      This study aimed to build up a collection of field isolates of Porcine circovirus type 2 (PCV2) circulating in pigs of Viet Nam. This is the first study for development of vaccine against post-weaning multi-systemic wasting syndrome, one of the most important Porcine circovirus type 2-associated diseases. Seven isolates of PCV2 were selected as candidates for subsequent evaluations. The purity test showed that 100% of PCV2 isolates were free of CSFV, PRRSV, PPV, PEDV, TGEV, PRV, PAdV and Mycoplasma. However, there were two out of seven isolates were contaminated with PCV1, and thus were not eligible for the production of master seed. The titers of five pure PCV2 isolates were in the range from 102.00 TCID50/ 0.1 ml to 105.75 TCID50/ 0.1 ml, of which the titers of three isolates were higher than 105.00 TCID50/ 0.1 ml.

điểm /   đánh giá
Published
2017-09-27
Section
ANIMAL AND AQUACULTURE SCIENCE, VETERYNARY