IDENTIFYING MAP LOCATION AND MARKERS LINKED TO THERMOSENSITIVE GENIC MALE STERILITY GENE IN 103S LINE

Abstract

     The thermosensitive genic male sterile rice line, TGMS 103S, is popularly used in the rice hybrid seed production in North Viet Nam. It is well known as the mother line of national hybrid rice varieties, such as Vietlai 20 and Vietlai 24. Genes controlling sterility of the TGMS 103S were not determined. The objective of this study was to identify genes controlling f sterility of TGMS 103S by using DNA markers. Phenotypic and genetic analyses were conducted by using F1 and F2 population derived from TGMS103S and R20. Linkage groups and the order of markers were determined using MAPMAKER//EXP 3.0. The phenotypic and SSR data were combined for linkage analysis using the MAPMAKER program and a linkage map of specific chromosome region surrounding the tgms gene was constructed. The result showed that tms-103S of TGMS 103S was located on chromosome 2 with flanking SSR markers of RM3294, RM6378, RM7575 and RM71. These markers could be used for marker-assisted selection/marker-assisted backcrossing to transfer tms-103S for developing female parent in two line hybrid rice breeding.