DETERMINATION OF VITAMIN C IN BROCCOLI, ORANGE JUICE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

An analysis method of vitamin C, including L-ascorbic and dehydroascorbic,
in broccoli and orange juice was investigated. The sample was extracted using
meta-phosphoric acid 20g/L, analyzed on a high-performance liquid
chromatography (HPLC) system, and performed by AOAC techniques. The limit of
detection (LOD) was determined at 4.31mg/100g with broccoli and 3.24mg/100g
with orange juice. The efficiency of recovery was found at 87.6 - 105.4% with
broccoli, and at 88.3 - 97.7% with orange juice. Consequently, the vitamin C
extraction using meta-phosphoric acid and the detection by HPLC is a highly
stable, efficient method.