INVESTIGATE THE ANTIOXIDANT AND ANTI-OSTEOPOROSIS ACTIVITIES OF COMPOUNDS ISOLATED FROM CHRYSANTHEMUM INDICUM FLOWERS

Abstract

In this research, five compounds were isolated from Chrysanthemum indicum L flowers and
determined their structures to be Syringin (1), Dihydrosyringin (2), Benzyl-O-β-D-
glucopyranoside (3), -Phenylethoxy-O--D-glucopyranoside (4), and (Z)-5’-Hydroxyjasmone
5’-O-β-D-Glucopyranoside (5) by analysis 1D - NMR spectra and compared to literatures. These
compounds were investigated antioxidative and anti-osteoporosis activities. Among them,
compound 5 showed the best antioxidant activity at a concentration of 10µM with the value of
peroxyl radical scavenging to be 11.39 ± 0.11 times higher than the protection provided by 1.0
μM Trolox and compound 2 exhibited meaningful reducing capacity, with CUPRAC values of
11.24 ± 0.01 at 10µM. Compound 5 also exhibited the best anti-osteoporosis effect at a
concentration of 10.0µM with percent of inhibition on RANKL-induced osteoclast differentiation
of 151.16 ± 13.13%.