STUDY ON SIMULTANEOUS DETERMINATION OF SOME FLAVONOIDS IN VIETNAMESE ORANGE JUICE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

The capabilities of photodiode array detection techniques were investigated for the high performance liquid chromatographic determination of 4
flavonoids including Naringin, Hesperidin, Neohesperidin and Quercetin in orange juice. The sample was dissolved in methanol, filtered and then pumped
into the LC: Waters Bridge C18 column (4.6mm x 150mm; 5µm), mobile phase including 0.1% phosphoric acid and ACN uses gradient program, 285nm
wavelength detector. The linearity of four substances in the range of 0.1 - 200mg/L with 0.9995 ≤ R2
≤ 1. MDL and MQL respectively of naringin, hesperidin,
neohesperidin, quercetin were 0.12mg/L and 0.40mg/L; 0.17mg/L and 0.57mg/L; 0.13mg/L and 0.44mg/L; 0.18mg/L and 0.61mg/L. The validated method
was applied to simultaneously determine naringin, hesperidin, neohesperidin, and quercetin in 45 Vietnamese orange juice samples. Naringin,
neohesperidin, and quercetin were not detected in the Vietnamese orange samples. The content of hesperidin varied depending on the orange variety and
region. The highest in oranges grown in Quang Ninh.