EXPRESSION OF MATRIX 1 PROTEIN (Ml) GENE OF INFLUENZA VIRUS A/H5Nl IN ESCHER1CH1A COLI

  • Trần Ngọc Tân

Abstract

Influenza is a dangerous disease caused by viral infections, especially influenza A virus. The danger lays in its capability to promote pandemic outbreak in both human and animal. The clinical symptoms of influenza diseases are similar to that of other viral or bacterial diseases. It makes difficulty to recognize the real disease. Therefore, the creation of simple and efficient method for detection of influenza A virus is necessary. Matrix protein M I of influenza A virus was used as the specific antigen for distinguishing influenza A virus from others such as influenza Band C. In order to produce recombinant protein M I, we selected gene All coding for M I antigen to express in E. coli. After expression, antigenic recombinant protein MI was purified by His-tag affinity column. The assay of immune binding between the recombinant antigen with natural antibody by Western blot technique has shown that the recombinant antigen (45 kDa) bound specifically to natural antibody raised against H5Nl. The result indicates that, protein M 1 can be applied to produce diagnostic Kit for detecting influenza A virus.    

Tác giả

Trần Ngọc Tân
điểm /   đánh giá
Published
2011-11-30
Section
Articles