INHIBITORY ACTIVITY AGAINST VIBRIO IN SHRIMP FARMING WATER OF BACILLUS SUBT/LIS HYI AND LACTOCOCCUS LACTIS CC4K

  • Đặng Phương Nga

Abstract

Lactic acid bacteria and Bacillus subtilis are widely used as probiotic for human and animal in order to improve the host digestion. However, in probiotic for shrimp pond water treatment, these bacteria may play different roles as competitor and inhibitor of pathogenic bacteria. Bacillus sp. strain HY I and lactic acid bacterium, strain CC4K isolated from shrimp pond water and fermented cabbage, showed inhibitory activity against Vibrio parahaemolyticus HHl, V. fUrnisH VT14-1, V. harvey NC5-1 and V. vulnificus HH2. Strain HY 1 showed inhibitory activity against Staphylococcus aureus, Sarcina lutea, Bacillus subtilis. Candida albicans, Aspergi/us sydowi, A. ustus, A. kilience. but no inhibitory activity against Escherichia coli, Saccharomyces cerevisiae and Penicillium oxalicum. Strain CC4K exhibited inhibitory activity against only some prokaryotic microorganisms as S. aureus, S. lutea. B. subtilis and was not capable of inhibiting eukaryotic microorganisms. Classification of these strains based on their morphological, physiological characteristics and 16S rRNA analysis revealed that they are B. subtili • HYI and Laefoeoeeus laelis CC4K with 99% and 99.1 % identity, respectively. Thin layer chromatography and infrared analysis showed that the antibacterial compound produced by strain CC4K might be lactic acid. The antimicrobial activity of strain HYI was due to an extracellular nonpolypeptide antibiotic. Because of their antibacterial activity. the two strains currently used as probiotic for shrimp pond water treatment at some locations in Vietnam.

Tác giả

Đặng Phương Nga
điểm /   đánh giá
Published
2011-11-29
Section
Articles