SCREENING AND INVESTIGATING EFFECTS OF CULTIVATION FACTORS ON THE CELLULASE PRODUCTION BY PENICILLIUM SP. DTQ-HK1

  • Trịnh Đình Khá
  • Quyền đình Thi

Abstract

Among 49 microbial strains isolated from waste composting, soil, straw, waste water and tissue, 36 strains showed cellu[ase activity. Three fungal strains RMl.1, RMI 5, HK[ indicating highest activities were selected for study. Alignment of 28S rRNA fragments indicated that strain RMl.1 had [00% homology with Aspergillus jumigalus strain ATCC [6907, strains RM [.5 and HK[ had high homology to some representatives of genus Emericella (99.8%) and genus Penicillium (98.6%), respectively. The 28S rRNA fragment of these strains were deposited in OenBank with accession number EFO [2766 (Aspergillus jumigalus strain DTQ-RMl.1), EF025927 (Emericella sp. DTQ-RM1.5), and EF087978 (Penicillium sp. DTQ-HK[). Penicillium sp. DTQ-HK[ showed maximal cellulase activity (1.58 U/ml) after [20 hours of cultivation at 30°C, pH 5.5, in the CPY medium containing 0.5% paper powder. Soybean powder and straw were suitable nitrogen and carbon sources for maximal cellulase production, respectively. A simple and locally-available medium for [arge-scale fermentation contained per liter: 5 g cellulose powder, [6 g soybean, 6 g straw, [ g K,HPO" 0.5 g MgSO" 0.5 g KCI; IO mg FeSO" pH 5.5. [n this medium, cellulase production was three times (5.09 Ulml) as high as that in the CPY medium (1.58 U/ml)

Tác giả

Trịnh Đình Khá
Quyền đình Thi
điểm /   đánh giá
Published
2011-11-29
Section
Articles