CLONING OF GENE ENCODING THE GROWTH HORMONE FROM COMMON CARP (CYPRINUS CARPIO)

  • Thẩm Thị Thu Nga

Abstract

Growth hormone plays an important role on fish physiology and metabolism. Recombinant growth hormone of human and some animals have been produced for a variety of aims such as: research, medicine, agriculture and fishery. In order to produce transgenic fish, especially in autotransgenic strategy, which is considered a best method for exploiting transgene, and fish growth hormone for aquaculture, growth hormone gene from common carp (Cyprinus carpio) was cloned and sequenced. Total RNA extracted from common carp pituitary gland was used as template for reverse transcription - polymerase chain reaction (RT-PCR). A pair of primers was designed base on M27000 sequence in GenBank Nucleotide Sequence Databases. RT-PCR product of 648 bp DNA, was cloned into pCR2. TOPO cloning vector. Its sequence showed 99.38% homology to the mRNA sequence in GenBank with accession number M27000. This structure gene will be expressed in E. coli, then be bound with common carp p~actin promoter to make "all fish" GH transfergene construct for autotransgenic fish

Tác giả

Thẩm Thị Thu Nga
điểm /   đánh giá
Published
2011-11-29
Section
Articles