DEVELOPMENT OF HBcAg COATED LATEX PARTICLE FOR DETECTION OF ANTI-HBcAg ANTffiODY IN HBV INFECTED PATIENT'S SERUM

  • Chu Hoàng Hà

Abstract

Hepatitis B is the most common serious liver infection in the world. It is caused by the hepatitis B virus (HBV) that attacks liver cells and can lead to liver failure, cirrhosis or cancer of the liver. The virus is detected by many rapid tests based on agglutination or immunochromatographic principles. A rapid recombinant antigen-based latex agglutination test (LA T) has been developed successfully to detect specific anti-HBc antibodies from human sera. The purified recombinant HBc antigen was attached to carboxyl-modified microspheres via covalent coupling. The agglutination between the recombinant HBc antigen-coated latex beads and anti-rabbit serum was evaluated before these latex beads were tested with human sera. Latex beads coated with 400 J.lg of the recombinant HBc antigen can detect antibodies more sensitively than the 100 J.lg, 200 J.lg-coated latex beads. The new diagnosis test employing HBcAg coated latex beads was sensitive, specific and accurate as compared to the standard lateral flow test and ELISA test. The synthesized HBcAg coated latex beads are stable and could be stored at 4'C for more than three months without loosing activity. Moreover, the recombinant HBc antigen-coated latex beads could detect specifically anti-HBc antibodies during chronic phase of the illness. The test is simple, inexpensive, and less time-consuming in the management of a large number of patients.

Tác giả

Chu Hoàng Hà
điểm /   đánh giá
Published
2011-11-29
Section
Articles