GENETIC DIVERSITY IN SOYBEAN GENOTYPES USING RAPDs AND SSRs

Abstract

Two sets of 30 soybean accessions developed by Cuulong Delta Rice Research Institute and Institute of Agricultural Sciences for Southern Vietnam were analyzed to determine DNA fingerprings using peR - based markers. In this study, random amplifed polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers were used to investigate relationships among 30 varieties. After screening and analyzing with 13 RAPD markers and 8 SSR markers, all RAPDs and six polymorphic SSRs giving the best results were addressed. A total of 6 SSR bands and 9 RAPD marker sites were used to calculate Jaccard's distance coefcients for cluster analysis using a unweighted pair-group method and an arithmetic averaging (UPGMA) algorithm. The higher discriminatory power of SSRs over RAPDs were recognized in the analyse. The genetic relationships identifed by SSR and RAPD were highly concordant, such that the correlation between SSR and RAPD genetic distance estimates was r ~ 0.79. The SSR and RAPD analysis of 30 accessions allowed for their grouping into three distinct groups. Dendrogram constructed by NTSYSpc program clearly separated 30 samples into three groups according to species. This would provide good infonnation for breeding materials in the future.