THE EFFECT OF GAMMA IRRADIATION ON REGENERATION ABILITY OF IRRADIATED RICE CALLI AND MOLECULAR ANALYSIS OF REGENERATED PLANTS

Abstract

The drought of agricultural area causes a great loss of crops. Production of drought resistant rices is a practical sense requirement. Many mutated genes were selected and effectively used in breeding programs. To contribute to the production of materials for selection of drought resistant rices, we have carried out the artificial mutation, in an attempt to produce drought resistant rice by y-irradiation of calli and regeneration of plant from irradiated calli. In this paper, we present the results on the effect ofy-ray doses on plant regeneration from irradiated calli and the results on molecular analysis of regenerated plants. Three-week old calli derived from seed embryos of two rice varieties: Chi chua I and C71 were exposed to 0 to 5 krad of y-rays. The alteration of genome of regenerated rice lines were analyzed using RAPD and SSR techniques. The obtained results showed that plant regeneration ability has been reduced by y-ray, the most clear reduction was observed at 5 krad. The results on RAPD analysis indicated that there were alterations in the genome of the obtained rice lines. Based on the results from the analysis of five SSR markers (RM121, RM242, RM263, RM250, RM270) linked to root traits favorable for drought resistance, eleven rice lines which may resist to drought were selected.