STUDY ON PLANT REGENERATION AND TRANSFORMATION SYSTEM OF SOME VIETNAMESE TOMATO CULTIVARS (LYCOPERSICON ESCULENTUMILL.)

  • Đỗ Xuân Đồng

Abstract

A comprehensive transfonnation system in plant mediated by Agrobacterium tumefaciens nowadays allows to rapidly improve the yield and quality of many crop plant species. In this study, a regeneration system for Vietnamese tomato cultivars has been developed employing multishoot generation from cotyledons. The results showed a high regeneration rate for three studied cultivars between 78 and 92% on MS + 2 mg!1 ZR media (cotyledons must be transfered into media containing I mg/I ZR after two weeks). However, the highest rate 95% was obtained for PTIS cultivar on media containing MS + folic acid 50 mg!1 + biotin 5 mg/I + I.S mg/I ZR + 20 g saccharose + S g agar. After seven weeks, cotyledons could develop into complete new shoots. The shoots had been subsequently transferred into media (MS + folic acid 50 mg/I + biotin 5 mg!1 + 0,3 mg/I IAA) to promote development of root system. After two weeks, an average number of 14 ± 2,08 roots per shoot had been achieved. Finally, successful plant would be cultivated on mixture of 40% soil, 30% rice husk and 30% organic fertilizer. This regeneration system has been used for developing a gene transformation protocol of tomato. At first, the  otyledons of PTIS cultivar were inoculated with A. tumefaciens LBA 4404 strain carrying pBII21 vector which contains reporter GUS gene. After that, these cotyledons must be transferred into regeneration media supplements with 50 mgtl kan for selection and subsequently into root induction media for nine weeks. All of completely developed plants were checked by X-glue staining to confirm the presence of GUS gene. Results indicated that 13.6% of plants have been successfully carried GUS gene

Tác giả

Đỗ Xuân Đồng
điểm /   đánh giá
Published
2011-11-29
Section
Articles