DETERMINATION OF RECOMBINANT INTERLEUKIN-2'S ACTIVITY BY EMPLOYING OF THE CELL LINE CTLL2

  • Đỗ Thị Thảo

Abstract

Produced by T Iympho cells of the immunological system, interleukin-2 (lL-2), a cytokine is being used widely in medicine. One of the most popular applications of IL-2 is for cancer treatment. Based on modem gene technique, several recombinant IL-2 products with good quality under different trademarks are manufactured. In order to investigate the bioactivity of IL-2 samples, we selected the CTLL2 cell line purchased from ATCC (American Type Culture Collection) in US. CTLL2 are the cells that completely depend on IL-2 to survive. Based on the ability to maintain and stimulate the CTLL2's growth of studied IL-2 samples, the bioactivity was determined. By applying the bioassay set up with the basement of the above character of CTLL2 cells, we chose 3 samples which showed the most active in our bioassay system for further bioactivity detennination after preliminary screening 15 samples. In result, the sample named as M3 possesses the highest activity (2.7 x 10' Units/mg). This activity is equal with that of IL-2 product from China (2.9 x 10' Units/mg) but lower than that ofIL-2 product from US (2 x 107 Units/mg).

Tác giả

Đỗ Thị Thảo
điểm /   đánh giá
Published
2011-11-29
Section
Articles