CLONING AND SEQUENCE ANALYSIS OF THE SUCROSE SYNTHASE PROMOTER (RSUCI-PROMOTER) FROM VIETNAMESE RICE CUL TIVARS

  • Lê Thị Thu Hiền

Abstract

The promoter region of rice sucrose synthase 1 gene (Rsuc1-promoter) was known as a phloem specific promoter. To study the diversity on these promoter regions from Vietnamese rice cuitivars, Tam Xoan Hai Hau and Du Thorn, Rsucl-promoters were amplified and cloned into pJETI cloning vector. Nucleotide sequences analysis of the recombinant plasmids showed an additional fragment of 13 nucleotides from Vietnamese cultivars as compared to those of Japanese cultivars. Comparison of nucleotide diversity in two above cultivars and C71 cultivar revealed a much higher level of nucleotide substitutions in the Du Thom (18 positions) than that in Tam Xoan Hai Hau (8 positions). Besides constitutive regulatory elements, TAT A and CCAA T boxes, these promoters contain also phloem specific regulatory elements such as ASL, 11 and GATA boxes. Therefore, these sequences can be used in transgenic plants to obtain high levels of expression of foreign and endogenous genes in phloem. In addition, these sequences may be useful for the production of genetically modified crops containing insect resistant genes which have agricultural values. The Rsuc I-promoter sequences from Tam Xoan Hai Hau and Du Thorn were submitted on 02-MA Y -2007 to the EMBLIGenBank/DDBJ databases (Accession numbers EF633717 and EF63371S, respectively).

Tác giả

Lê Thị Thu Hiền
điểm /   đánh giá
Published
2011-11-28
Section
Articles