EXPRESSION NAE GENE ENCODING NEURAMINIDASE ANTIGENIC DETERMINANT OF INFLUENZA VIRUS AlH5Nl IN E. COLI

Abstract

Neuraminidase is one of the main influenza A surface antigens. Having the length of 450 amino acids, NA has both portion enzymatic and antigenic properties. After analyzing NA amino acid sequence, the gene encoding NA epitope antigen protein (Nae) was cloned. This gene fragment was amplified by PCR with specific primer pairs. Using QIA-quick column to purify the PCR product, Nae gene was excised by restriction enzymes HindllI and Xhol and ligated into the pET22-trx vector then transformed into the E. coli BL21 host. After fusion Nae protein was expressed, the antigenic ability to match the natural NA (NI) antigen was determined by Western blot technique. The result of Western blot showed that Nae recombinant protein has specific binding ability to natural antibody against H5N 1. With this result, Nae protein is promising to be develop to a s supplemented product for a vaccine against avian influenza virus H5N 1.