GENETIC RELATIONSHIP OF RICE GRASSY STUNT VIRUS STRAINS ISOLATED FROM THE SOUTHERN CENTRAL PROVINCES

  • Nguyễn Ngọc Sơn

Abstract

From the year 2006 - 2008, the situation of infected pests and viruses causing destruction on rice has become a danger to the Cuu Long River Delta (CLRD), where rice output is the largest nationwide. According to the latest figures in 2008, on the autumn-winter rice in provinces of CLRD, the total pestinfected area are is 25,198 ha, of which the area of virus-infected rice is 3546.8 ha. Viral disease caused by the combination of from one to three types of viruses, including: Rice Grassy Stunt Virus (RGSV) belonging to Tenuivirus, Rice Ragged Stunt Virus (RRSV), the standard species of Oryzavirus, and Rice Tungro Spherical Virus (RTSV) belonging to Waikavirus. In 2007, the genetic variations of some virus strains in CLRD provinces have initially been investigated and evaluated. In this current study, for the first time, the rice samples suspected with viral infection in the provinces of Southern Central area have been collected to test the presence of the virus in this region, contributing to the forecast, updated figures and the strategy coping with this disease that tends to spread in the Southern Central area. Using RT-PCR with specific primers for RGSV, RRSV and RTSV, we have detected the presence of NCP-coding gene of ROSV in 2 provinces Ninh Thuan and Binh Thuan in total 5 investigated provinces. However, we do not see the presence of RRSV and RTSV in this area. The high conservation of nucleotide sequence of RGSV in 2 provinces of Southern Central area compared with virus strains in CLRD showed that RGSV strains appearing in Southern Central area could be originated from the CLRD area

Tác giả

Nguyễn Ngọc Sơn
điểm /   đánh giá
Published
2011-11-25
Section
Articles