EFFECTS OF CO, CONCENTRATIONS AND LIGHT INTENSITIES ON THE GROWTH OF IN VITRO AND EX VITRO STRAWBERRY PLANT LETS (FRAGARIA ANANASSA OUCH.)

Abstract

Fragaria ananasa Ouch. shoots with three leaves and 80 mg of weight were cultured in vitro for 4 weeks on MS sugar-free medium with different light intensities (100 μlmol/m²/s and 200 μlmol/m²/s) under CO2 enriched (1000 μlmol) and non-enriched (400 μlmol) conditions. In vitro plantlets were then transferred to the ex vitro condition for 4 weeks. Under the in vitro CO2 enrichment the growth shown by fresh weight of root, shoot, plant and total dry weight of in vitro plantlets increased, significantly. The growth of in vitro plantlets was the highest in high light intensity conditions. The fresh weight, length and number of runner were highest in the CO2 enriched and high light intensity. There was difference no significant in the percent age of dry matter of ex vitro plantlets among treatments on days 14,21 and 28.