AN EFFICIENT PROTOCOL FOR PLANT REGENERATION OF XOAN TREE (MELIA AZEDARACH L.) VIA SOMATIC EMBRYOGENESIS INDUCTION

Abstract

A system for callus induction from hypocotyl and regeneration of the persian lilac tree or xoan in Vietnameses (Melia azedarach L.) via somatic embryogenesis has been optimised. Research results show that the rate of caUus formation on CL media (MS supplemented with 3 mg/I NAA and I mg/I BAP) is 92.2%. The callus clusters were subsequently transferred onto SE3 media (MS supplemented with 11 mg/I BAP + 60 g saccharose) for somatic embrogenesis during the period from 4 weeks the number of embryo as well as embryo quality is the best and the  average ratio was 12.7 embryos per explant. Embryoclusters were continue transferred onto RE media for shoot maturation. Complete developed shoots with length of about 0.7 - 1.5 em were transferred onto R media (MS supplemented with 3 mg/I IBA) for 3 days and rooted on MS without plant growth regulator for 4 weeks with the rate of tree roots out of 100%. Complete plantlets were transferred to greenhouse and cultivated on a mixture rice husk and sand under ratio 4:6. The protocol required 18 weeks in order to produce regenerated plants and the process is entirely appropriate for further genetic transformation experiments