CLONING OF HYBRIDOMAS PRODUCING MONOCLONAL ANTIBODIES AGAINST THE VP28 OF WHITE SPOT SYNDROME VIRUS IN SHRIMP

Abstract

Production of monoclonal antibody for early specific diagnosis of human and animal's diseases is not a new application. However, as the urst case in Vietnam, we were successful in fusion and cloning of 5 clones of hybrid om a which were able to produce specific monoclonal antibodies against VP28 - a viral envelope protein of WSSV in shrimp under concentration of immunogen as 0.05 mg/ml. The concentrations of the 5 monoclonal antibodies purified from supernatants of the cultured 5 clones of hybridoma ranged from 54 - 95 μg/l and working dilutions of those ranged from 1,000 - 3,500 respectively. All monoclonal antibodies showed positive results after ELISA checking on WSSV infected shrimp samples. However, those monoclonal antibodies still need to be characterized in our further research.