ISOLATION PSEUDOMONAS STUTZERI FROM SOILS OF THE MEKONG DELTA, VIETNAM

Abstract

Twenty isolate of Pseudomonas stulzeri were isolated from three kinds of soil in the Mekong Delta based on the protocol have been previously reported and twelve isolates were identified by 16S rRNA technique with universal primers (1.5 kb ladder) together with positive strain (ATCC 14405). One isolate (P8 isolate) was identified with specific primer at 445 bp ladder. The pertial 16S rONA gcne of this isolate was sequenced and compared with bacterial 16S rONA genes in GeneBank by BLAST N sofware and the result showed that the sequence of P8 and that of Pseudomonas stutzeri with accession number Y 18006 were 99% identical