GENETIC POLYMORPHISMS IN THE MATRIX METALLOPROTEINASE-2 (MMP-2) PROMOTER IN SOME NASOPHARYNGEAL CARCINOMA PATIENTS

  • Nguyễn Thị Ngọc Hà

Abstract

Matrix Metalloproteinase-2 (MMP-2) has been shown to play an important role in cancer invasion and metastasis because of their capability to degrade or break down both extracellular matrix and basement membrane. According to recent studies, single nucleotide polymorphism (-1306C/T) in MMP- 2 promoter abolishes the Sp I-binding site, a transcription factor, and thus may down regulate expression of gene MMP-2. In this study, we analyzed polymorphisms in the MMP-2 promoter in Some Vietnamese nasopharyngeal carcinoma (NPC) patients, approaching the association between the polymorphisms in MMP-2 promoter and the metastasis of NPC. Total genomic DNA was isolated and purified from the paraffin block of9 NPC patients and 3 blood samples of healthy people. MMP-2 promoter was amplified by using a pair of primers MMP-2F and MMP-2R. The purified recombinant plasmids were used for sequencing of the inserts in both directions. The result is that excepting -1306C/T in MMP-2 promoter was reported in GenBank, we found four sites -1235TIC, -I 339TIC, -1440T/C and -1456T/C that have been not public in any database. Perhaps, these are polymorphisms in the MMP-2 promoter region of the Vietnamese nasopharyngeal carcinoma patients that may result in changes of the expression of gene MMP-2, one of key factors related to the metastasis of NPC

Tác giả

Nguyễn Thị Ngọc Hà
điểm /   đánh giá
Published
2011-11-23
Section
Articles