POLYCYCLIC AROMATIC HYDROCARBON BIODEGRADATION OF BACTERIAL STRAINS ISOLATED FROM HERBICIDE/DIOXIN DETOXIFICATION BIOREACTOR
Abstract
From detoxification tteatment of herbicide/dioxin contaminated soil in semianaerobic bioreactor, four chrysene degraders and two pyrene degraders were isolated. Among these bacterial strains, only two sfrains, BDNRl and BDNR4, were able to utilize soil exfraction containing 2,3,7,8-TCDD and 2,4-D, respectively. Both bacterial sttains belong to Gram negative. BDNRl and BDNR4 degraded seven different PAHs such as phenanthrene, anthracene, fluoranthene, naphthalene, fluorene, pyrene and chrysene. After 7 days of
incubation, BDNRl degraded 86.2% pyrene, 50% anthracene, 50% naphthalene, 50% phenanthrene, 44.4% fluoranthrene and 20% fluorene; BDNR4 bacterial strain also degraded 61.5 % pyrene, 50% anthracene, 50%
naphthalene, 65.3% fluoranthrene, 47% fluorene and 23.1% naphthalene. Based on several morphological properties and partial sequence of 16S rRNA gene, BDNRl sfrain should belong to the genus Pseudomonas
and named as Pseudomonas sp. BDNRl. The partial sequence of 16S rRNA gene ofthe sfrain BDNRl was deposited in Genbank with an assession number EU726633.