ANTHER CULTURE FOR PRODUCTION OF HOMOGENEOUS LINES IN THE TU LE TRADITIONAL GLUTINOUS RICE

Abstract

Tu Le variety is a ttaditional glutinous rice in Tu Le valley. Van Chan disttict. Yen Bai province. This variety has many precious characteristics: high and long glutinosity, pleasant aroma and can stand well with sttess. It is a precious gene source that needs exploiting. However its degeneration causes the reduction of quality and productivity. In this paper, the anther culture technique was applied for improvement of this variety. 8640 anthers were cultared, 684 cali were produced in Cil and C12 medium. Call were obtained in

both media gave a high plant regeneration frequency in PR medium: CII (60.87%)) and CI2 (30.76%). Sixty seven green plant lines were fransferred to green house. In these lines, 45 were seeded (67.16%) and 22 were haploids based on morphological appearance. The analyses of 45 seeded lines with 8 primer pairs related to amylose content and aroma showed that only waxy gen (Wx) encoded granule-bound starch synthase was altered in 2 lines. All 45 lines were homogeneous in 7 loci generated by remained 7 primer pairs. These lines will be maintained for flirther stadies on homogeneity and stable agronomic characteristics.