Effect of natural auxin from Portulaca grandiflora hook and Ipomoea batatas (l.) poir on the formation adventitious roots in vitro of Plumbago zeylanica l.

Abstract

Plumbago zeylanica L. is a traditional herbal that has been reported to treat on skin diseases. Furthermore,some researchers have found plumbagin extracted from roots of this species can prevent cancer cell development. Incurrent study, stems of Plumbago zeylanica L. were cultured on MS medium with BA 1.0 mg/L and IAA (0.01-0.15mg/L) or NAA (0.1-0.15 mg/L). After 8-week cultured, stems were transferred to MS medium with extracted fromstems of Portulaca grandiflora Hook (2-10 ml/L) or extracted from stems of Ipomoea batatas (L.) Poir. The resultsshowed that, the appropriate medium for shoot formation was in MS with BA and IAA 0.1 mg/L or NAA 0.1 mg/L.The adventitious roots in vitro were formatted in MS medium supplied with extracted from stems Portulacagrandiflora Hook or from stems of Ipomoea batatas (L.) Poir 6 ml/L. Simultaneously, after 8-week cultured, theadventitious roots were collected and plumbagin qualitative were analyzed with pure plumbagin of Sigma. As theresults, plumbagin presents in adventitious roots cultured.