Application of Enzyme - Linked Immunosorbent Assay method to analyse quinolone residues in shrimps coming from the North Vietnam

  • Phạm Kim Đăng

Abstract

A commercial Enzyme-Linked Immunosorbent Assay (ELISA) provide by CER Belgium was evaluated for the analysis of quinolone antibiotics in shrimps coming from Vietnam. The kit validation study was performed according to the Commission Decision 2002/657/EC criteria established for semi-quantitative methods. Quinolone residues were measured in 90 shrimp samples coming from Hanoi, and provinces of Quang Ninh, Nam Dinh and Nghe An. Screening was performed with ELISA and confirmation by the LC-MS/MS. Result showed that the kit is suitable for analysis of all quinolone antibiotics in shrimp tissue with a limit of detection level of 0,7 ppb. Residues of 5 quinolones (enrofloxacin, norfloxacine, ciprofloxacin, danofloxacin and oxolinic acid) were found in 9 samples (10 %), with levels ranging from 0,4 to 145 ppb. Four samples displayed a residue level higher than the Maximum Residue Limit fixed by European Union in directive 2377/90 CE
điểm /   đánh giá
Published
2012-11-20
Section
Bài viết