Effect of Different Media and Some Factors on Efficiency of Anther Culture in Indica Rice

Abstract

Anther culture is a rapid method of producing doubled haploid lines as compared with pure-line development by conventional self-pollination. However, the efficiency of anther culture depends upon several factors: genotype, temperature and light during culture, medium composition and treatment of plants prior to culture. Four basic media were used, i.e. MS, M-019, N6 and SK-1 with agar concentration of 0.5%; 0.75% and 1.0% and concentration 60g/l and 80g/l of maltose and sucrose as carbon source. The results indicated that the N6 and SK-1 media were better than MS and M-019 for improving callus induction in rice. The concentration of 60g/l was notably better than that of 80g/l when using sucrose or maltose as carbon source. Meanwhile, using 0.75% of agar obtained the highest induction efficiency. Callus transfer time showed remarkable improvement of plant
regeneration. We found that 5 days and 10 days, yielded low number of albino plants compared to 15, 20 days. This result indicated that if callus kept for longer period in induction medium then regeneration efficiency, especially of green plants dramatically reduced and but the rate of albino
plants increased.