Selection of Optimal Conditions to Produce Chitosanase from Streptomyces griceus (strain NN2)

  • Ngô Xuân Mạnh

Abstract

Chitosanase hydrolyzes chitosan to produce chitosan oligosaccharide (COS) which is applied widely in industry. To select the best train of microorganisms and optimal conditions to produce chitosanase was the purpose of the present study. Strain NN2 of Streptomyces griceus was found the best to produce chitosanase among the strains isolated. Based on the cell growth curve, the time to reculture the strain should be afterabout 36 hours in  activatedculture. The best culture medium composition for the highest level of chitosanase was obtained with 0.3% molasses at a pH of 6.0, 37oC and 3 days of fermentation. Comparing two methods to partly purify chitosanase, viz. precipitating with ethanol and with amonium sunfate showed that amonium sunfate preciption was better and the optimal of concentration of amonium sulfate was 50 - 70%, which resulted in 1.8 fold purification and a chitosanase specific activity of 201.9 U/mg
điểm /   đánh giá
Published
2014-01-24
Section
Bài viết