Effect of Ethylmethane Sulphonate on In vitro Mutagenic Treatment of Carnation (Dianthus caryophyllus L.)

  • Nguyễn Thị Lý Anh

Abstract

This research aims at identifying mutagenic effect of ethylmethane sulphonate (EMS) on in vitro treatment of carnation. The stem segments bearing node of in vitro plantlets were soaked in EMS solution at different concentrations (from 0 to 1.0%) for 1 to 3 hours. After soaking, the explants were cultured on MS medium + 1 ppm kinetin for shooting and then transferred to MS medium + 0.5 ppm α-NAA for rooting. The results showed that the survival and regeneration rate of explants decrease with increasing EMS concentration and soaking time. EMS in vitro treatment resulted in increasing variants of in vitro shoots by 5.1 - 22.7 times in comparison with the control. The optimal EMS concentration and treatment duration is 0.4% and two hours, respectively. Five types of shoot variants appeared (A, B, C, D, E) and the growth rate, number of leaves and rooting ability decreased in following order: A > B > D > E > C. Based on the experimental data obtained, a formula expressing the dependence of the survival of the explants and the variant rate of the shoot on the EMS concentration and treatment duration was established, which can serve as basis for subsequent study on commutation treatment in carnation
điểm /   đánh giá
Published
2013-07-31
Section
Bài viết