STUDY ON SHOOT REGENERATION FROM LEAF CALLUS OF TETRACERA SCANDENS L.

Abstract

Tetracera scandens is an important herb, used to treat some diseases, such as gout, kidney diseases... The callus is formed from leaf on MS medium (Murashige & Skoog, 1962) supplemented with 2.5mg/l 2,4-D and 0.5mg/l BA. Before induced shoot regeneration, the callus had grown on MS medium supplemented with 0.5mg/l BA and 0.5mg/l GA₃. Shoot generation is actually induced on MS medium supplemented with 0.7mg/l BA and 0.1mg/l IAA. The number of shoot regeneration is about 26/callus (from 0,3cm² leaf tissue). Shoot origin, role of endogenous hormones and respiration rate in shoot regeneration were analysis.