Development of INCAT-GC-MS for analysis of metabolites in urine

  • Peter Podolec
  • Alexandra Hengerics Szabó
  • Viktória Ferenczy
  • Peter Kotora
  • Jaroslav Blaško
  • Róbert Kubinec
  • Stanislav Stuchlík
  • Martin Juriga
  • Radomír Čabala
  • Václav Bierhanzl
  • Ngô Mạnh Thắng

Abstract

This paper aims to develop a non-solvent microextraction technique using INCAT (Inside Needle Capillary Adsorption Trap) for GC-MS analysis of volatile metabolites in urine. INCATs with different sorbents were tested, resulting in an INCAT with 3 sorbent layers Chromosorb W+ 20% SE-54, Carbopack X and Carboxen 1000 (1 : 1 : 1), which proved to be the most suitable for differentiating and identifying volatile metabolites in urine samples of patients from those of healthy people.

điểm /   đánh giá
Published
2016-09-07
Issue
Vol. 19 No. 2K (2016)
Section
ARTILES
Copyright belongs to VNU-HCM “Science and Technology Development” Journal. Any copy or reprinting of any form must be permitted by the Journal.